Leica RM 2135

Rotary Microtome

Instruction Manual
Leica RM 2135 V1.1 English – 02/2001
Always keep this manual near the instrument!
Read carefully prior to operating the instrument!
1. Important information

The information, numerical data, notes and value judg-

Serial No. ..................................................................... ments contained in this manual represent the current
state of scientific knowledge and state-of-the-art tech-
Year of manufacture: ................................................. nology as we understand it following thorough investi-
gation in this field. We are under no obligation to up-
Manufactured in: .... Federal Republic of Germany date the present manual periodically and on an ongo-
ing basis according to the latest technical develop-
ments, nor to provide our customers with additional
copies, updates etc. of this manual.

For erroneous statements, drawings, technical illus-

trations etc. contained in this manual we exclude li-
ability as far as permissible according to the national
legal system applicable in each individual case. In par-
ticular, no liability whatsoever is accepted for any fi-
nancial loss or consequential damage caused by or
related to compliance with statements or other infor-
mation in this manual.

Statements, drawings, illustrations and other informa-

tion as regards contents or technical details of the
present manual are not to be considered as warranted
characteristics of our products. These are determined
only by the contract provisions agreed between our-
selves and our customers.

Leica reserves the right to change technical specifi-

cations as well as manufacturing processes without
prior notice. Only in this way is it possible to continu-
ously improve the technology and manufacturing tech-
niques used in our products.

This document is protected under copyright laws. Any

copyrights of this document are retained by Leica
Microsystems Nussloch GmbH.

Any reproduction of text and illustrations (or of any

parts thereof) by means of print, photocopy, microfiche,
web cam or other methods – including any electronic
systems and media – requires express prior permis-
sion in writing by Leica Microsystems Nussloch GmbH.

For the instrument serial number and year of manu-

Leica Microsystems Nussloch GmbH facture, please refer to the name plate at the back of
the instrument.
Heidelberger Str. 17-19
D-69226 Nussloch
Germany

Telephone: 0 62 24 / 143-0
Telefax: 0 62 24 / 143-200
eMail: [email protected]
Homepage: http://www.histo-solutions.com © Leica Microsystems Nussloch GmbH

Leica RM 2135 – Rotary Microtome 3

2. Table of contents

1. Important information ........................................................................................................................... 3

2. Table of contents ................................................................................................................................ 4
3. Safety instructions for handling the instrument ............................................................................. 5
3. Safety instructions for handling the instrument ........................................................................... 6
4. Technical data ....................................................................................................................................... 7
5. General description............................................................................................................................ 8
5.1 Leica RM 2135 - Overview ................................................................................................................... 8
5.2 General description .............................................................................................................................. 9
5.3 Standard delivery .................................................................................................................................. 9
6. Unpacking and installation ............................................................................................................ 10
6.1 Unpacking ............................................................................................................................................ 10
6.2 Site requirements ................................................................................................................................ 11
7. Setup .................................................................................................................................................... 12
7.1 Mounting the specimen clamping system ...................................................................................... 12
7.2 Mounting the accessories ................................................................................................................. 13
8. Operation ............................................................................................................................................ 14
8.1 Section thickness setting .................................................................................................................. 14
8.2 Coarse feed wheel .............................................................................................................................. 14
8.3 Specimen retraction ........................................................................................................................... 15
8.4 Mechanical trimming function (optional) ....................................................................................... 15
9. Specimen clamping system ............................................................................................................ 16
9.1 Mounting the specimen clamp ......................................................................................................... 16
9.2 Orienting the specimen ...................................................................................................................... 16
10. Universal knife holder base ............................................................................................................ 17
10.1 Knife holder base with lateral displacement ................................................................................. 17
10.2 Knife holder base without lateral displacement............................................................................ 17
10.3 Repositioning the knife holder base ................................................................................................ 17
10.4 Mounting the knife holder ................................................................................................................. 18
10.5 Adjusting the clearance angle ......................................................................................................... 18
11. Daily operation .................................................................................................................................. 19
12. Trouble shooting ............................................................................................................................... 20
12.1 Problems, possible causes and corrective action ........................................................................ 20
12.2 Instrument errors, possible causes and corrective action ......................................................... 21
13. Cleaning .............................................................................................................................................. 22
14. Maintenance ...................................................................................................................................... 23
14.1 General maintenance ......................................................................................................................... 23
14.2 Adjusting the spring lever system .................................................................................................... 24
15. Accessories........................................................................................................................................ 25
15.1 Specimen clamps................................................................................................................................ 25
15.2 Knife holders ........................................................................................................................................ 29
15.3 Section waste tray .............................................................................................................................. 34
15.4 Hand rest .............................................................................................................................................. 35
Product changes ................................................................................................................................. 38
Warranty ............................................................................................................................................... 38
Disposal ................................................................................................................................................ 38
Technical service information .......................................................................................................... 38

4 Instruction manual V 1.1 – 02/2001

3. Safety instructions for handling the instrument

3.1 Safety devices

The safety devices on the instrument and ac-

cessory equipment must not be removed or
modified!

The instrument incorporates the following safety de-

vices: quick locking system for the handwheel and a
knife guard on the knife holder.

3.1.1 Activation of the quick locking system of

the handwheel

Always lock the handwheel prior to manipu-

lating the knife and specimen or before chang-
ing the specimen or knife!
Fig. 5.1 1
The handwheel can be locked in any position using the
locking lever (1) on the right side of the microtome base
plate. The two locking positions of the lever (● = locked;
❍ = released) are marked on the microtome base plate
(Fig. 5.2).

Performance check

• To activate, pull the locking lever (1) to the front (l).

The handwheel is mechanically blocked and cannot

be turned.

• To deactivate, push the locking lever (1) to the rear

(m).

The handwheel can then be rotated again.

Fig. 5.2 1

3.1.2 Knife guard on the knife holder

2
1
2 Prior to manipulating the knife and specimen,
or changing the specimen or knife, and dur-
ing breaks, always lock cover the cutting edge
with the knife guard (1)!

Every knife holder has a permanent, movable knife

guard (1) that allows to cover the cutting edge com-
1
pletely in any position of the knife.
Grips (2) are provided to conveniently move the knife
guard. Push both parts of the knife guard to the middle
to cover the knife edge.

Fig. 5.3

Leica RM 2135 – Rotary Microtome 5

3. Safety instructions for handling the instrument

Transport and installation Cleaning

• Please refer to chapter 4 - 'Technical data'! • Before cleaning, activate the handwheel
locking system!
• The instrument must be transported in an
upright position! • Do not use solvents that contain acetone
and xylene!
• Do not carry the instrument with the handle
of the handwheel, coarse feed wheel or • Ensure that no liquids enter the interior of
control knob for section thickness setting! the instrument when cleaning!

• The safety devices on the instrument and • When using detergents please comply
accessory equipment must not be removed with the safety precautions of the manu-
or modified! facturer!

Operating the instrumentOperating the Maintenance

instrument • Only authorized and qualified service per-
• Take care when handling microtome sonnel may access the internal compo-
knives and disposable blades. The cutting nents of the instrument for service and re-
edge is extremely sharp and can cause pair!
severe injury!

• Never leave knives and knife holders with

a mounted knife or blade lying around! Al-
ways put the knives back into the knife
case when not in use!

• Do not place a knife on a table with the

cutting edge facing upward!

• Never try to catch a falling knife!

• Always clamp the specimen before the

knife!

• Prior to manipulating the knife and speci-

men, or changing the specimen or knife,
and during breaks, always lock the hand-
wheel and cover the cutting edge with the
knife guard!

• Do not attempt to clamp, approach or ori-

ent the specimen in the retraction phase
(while RETRACT illuminates)!
Prior to the subsequent section, a speci-
men advance will take place by the re-
tracted value plus the selected section
thickness.
This involves the risk that the specimen
collides with the knife!

• Always wear protective glasses when

sectioning brittle specimens!
Risk of splintering!!

• Ensure that liquids do not enter the inte-

rior of the instrument during work!

6 Instruction manual V 1.1 – 02/2001

4. Technical data

Type RM 2135

Operating temperature range: +10°C to +40°C

Microtome

Type RM 2135 Rotary Microtome

Section thickness setting 1 - 60 µm
from 1 - 10 µm in 1 µm increments
from 10 - 20 µm in 2 µm increments
from 20 - 60 µm in 5 µm increments
Horizontal specimen feed 30 mm (-10%)
Vertical specimen stroke 70 mm
Max. sectioning area without retraction 69 mm without specimen orientation 1 µm
Max. sectioning area with retraction 62 mm
Specimen retraction 200 µm
with ON/OFF

Optional accessories

Specimen orientation (option)

horizontal 8°
vertical 8°
rotation ±90°

Trimming steps (option) 10, 30 µm

Repositioning of knife holder base

Knife holder base with lateral displacement
North/south ±25 mm
East/west ±20 mm
Knife holder base W/O lateral displacement
North/south ±25 mm

Dimensions and weight

Side coarse feed Front coarse feed
Width 475 mm 415 mm
Depth 525 mm 565 mm
Overall height 295 mm 300 mm
Working height (knife edge) 105 mm 110 mm

Net weight: 34 kg
Weight (including accessories) 38 kg

Leica RM 2135 – Rotary Microtome 7

5. General description

5.1 Leica RM 2135 - Overview

Section thickness indication

Orienting Control knob for

specimen clamping system section thickness setting
(optional)

Universal cassette clamp

Knife guard

Coarse feed wheel Clamping lever

for
disposable blade
Knife holder E
for disposable blades

Clamping lever Section

for east/west movement waste tray
of the knife holder base

Hand rest
Fig. 8.1 (accessory)

Cover cap
for optional
illumination, magnifier/
Non-orienting
microscope attachment
specimen clamping system
(optional)

Standard specimen clamp Handwheel

Trimming lever
to activate
the trimming function
(optional)

Knife guard
Arm rests
Locking lever
for handwheel

Front coarse Locking lever

feed wheel for knife holder
(optional) and clearance angle setting

Clamping lever
for knife holder base
Fig. 8.2

8 Instruction manual V 1.1 – 02/2001

5. General description

5.2 General description 5.3 Standard delivery

The Leica RM 2135 is a manually operated rotary mi- The RM 2135 is available in six versions:
crotome.
The vertical and horizontal specimen movement takes 1. RM 2135 basic instrument with mechanical trimming
place via zero-backlash and maintenance-free cross function, lateral coarse feed, CW
roller guides. The coarse advance is manually oper- 2. RM 2135 basic instrument without mechanical trim-
ated by turning the coarse feed wheel. All components ming function, lateral coarse feed, CW
are well protected in a dustproof plastic housing. Sec- 3. RM 2135 basic instrument with mechanical trimming
tioning takes place by turning the extremely smooth function, lateral coarse feed, CCW
running handwheel. 4. RM 2135 basic instrument without mechanical trim-
The section thickness is selected via a control knob. ming function, lateral coarse feed, CCW
The mechanical trimming function (optional) is acti- 5. RM 2135 basic instrument with mechanical trimming
vated by means of a lever. function, front coarse feed, CW
Specimen retraction is a standard feature and may be 6. RM 2135 basic instrument without mechanical trim-
turned off when required. ming function, front coarse feed, CW

The instrument is designed and manufactured to con- The basic instrument is delivered with the following
form with the strict VDE safety standards. accessories:

- 1 Arm rest, right,

1 Arm rest, left,
- 1 Maintenance kit consisting of:
1 Allen key size 4,
1 Allen key size 3,
1 Screw driver,
1 Bottle of oil No. 405, 50 ml,
1 Dust cover,
- 1 Instruction Manual.

You will find these accessories as well as any further

accessories that you may have ordered, in the card-
board box on top of the instrument.

Compare the delivered components with the parts list

and your order. Should you find any discrepancies,
please contact your Leica sales office without delay.

Leica RM 2135 – Rotary Microtome 9

6. Unpacking and installation

6.1 Unpacking

Please check the Tip-n-Tell indicators

on the outside of the box upon arrival.
If the tip of the arrow is blue, the box was not
1
transported as required.
Please note this on the shipping documents
and check the contents for damage. If dam-
aged, make a complaint to the carrier imme-
diately.
2
• Cut off the steel bands on the outside of the card-
board box.
• Open the outer cardboard box (1).
3 • Take out the accessory box (2) and the instruction
manual.
• Remove the outer cardboard box (1) by lifting it up-
wards.
• Fold down the lateral parts of the inner cardboard
box (6).
• Take out the fixing module (3).

4 Do not carry the instrument with the handle of

the handwheel, coarse feed wheel or the con-
trol knob for section thickness setting!

• Lift the instrument (4) from the foam part (5) holding
it at the front of the base plate and at the recessed
5
grip at the rear (Fig. 10.2).

6
Installing the instrument
• Place the instrument on a stable laboratory table.

Two slide faces (7) located at the rear of the base plate
facilitate to reposition the instrument on the table.
Fig. 10.1

• To relocate, lightly lift the instrument at the front of

the base plate and slide it on the slide faces.

7
Fig. 10.2

10 Instruction manual V 1.1 – 02/2001

6. Unpacking and installation

6.2 Site requirements

The installation site must meet the following require-
ments:

- stable, vibration-free laboratory table,

- vibration-free floor,
- Obstruction-free access to the handwheel and
coarse feed wheel,
- Room temperature always between +10 °C and
+40 °C.

Leica RM 2135 – Rotary Microtome 11

7. Setup

The plastic grips of all clamping levers can 7.1 Mounting the specimen clamping system
be repositioned as preferred by the individual The basic instrument is delivered with an orienting or
user.
non-orienting specimen clamping system as ordered
Pull the grip out of the lever, hold it in this po-
sition, and turn it to the desired position. It will
which will have to mounted first.
then lock automatically when released.
7.1.1 Orienting specimen clamping system
• Lock the handwheel.

6
• To remove, turn the eccentric bolt (6) counterclock-
wise.
5 5a 5b 3 • Unscrew the thrust piece (5) with a screw driver
and pull it out together with the spring (5a) and pin
(5b).
• Unscrew the orienting screws (3) and (4) and re-
move.
• Place the orienting specimen clamping system (10)
on the microtome cylinder as shown.
7+8
2 • Introduce the 2 screws (7) into the holes (9a).
The 2 screws (8) are accessible through the holes
(9b).
1 • Tighten the screws (7) and (8) by using an Allen key
size 3.
4 • Insert the spring (5a) and the pin (5b) with the flat
9b end into the thrust piece (5) and screw down the
9b
7+8 thrust piece (5) with a screw driver.
10
9a • Screw down the orienting screws (3) and (4).
9a
Fig. 12.1
• Place the dovetail adapter (2) on the specimen
clamping system and fix it by tightening the 4 screws
(1) with an Allen key size 3.

To dismount, proceed in reverse order.

11 7.1.2 Non-orienting specimen clamping system

12 • Place the non-orienting specimen clamping system
(11) on the microtome cylinder as shown and fix with
the 4 screws (12) by using an Allen key size 4.
• Place the dovetail adapter (2) on the specimen
2
clamping system (11) and fix it by tightening the 4
screws (1) with an Allen key size 3.
1
To dismount, proceed in reverse order.

Fig. 12.2

12 Instruction manual V 1.1 – 02/2001

7. Setup

7.2 Mounting the accessories

The plastic grips of all clamping levers can

be repositioned as preferred by the individual
user.
Pull the grip out of the lever, hold it in this po-
sition, and turn it to the desired position. It will
then lock automatically when released.

7.2.1 Mounting the knife holder base

6 • To release, turn the lever (1) counterclockwise.

• Place the knife holder base (2) with the two clamp-
ing levers (3) and (4) mounted in place onto the T
5 piece (5) of the microtome base plate.

• To secure the knife holder base, turn the lever (1)

clockwise.
3

7.2.2 Mounting the arm rests

7 • Insert the pins on the underside of the arm rests (6)
in the holes (7) of the microtome base plate as
4 shown.

Fig. 13 2 For mounting the hand rest (optional acces-

sory), please refer to chapter 15 Accessories.

Leica RM 2135 – Rotary Microtome 13

8. Operation

8.1 Section thickness setting

The section thickness is selected with the control knob
(1) which is located on the right at the front of the mi-
crotome.

The control knob has a lock-in position for each incre-

2
ment.

Setting range: 1 - 60 µm
from 1 - 10 µm in 1 µm increments
from 10 - 20 µm in 2 µm increments
from 20 - 60 µm in 5 µm increments

The selected section thickness is indicated in the dis-

play window (2).

8.2 Coarse feed wheel

1 The instrument is available with side coarse feed (Fig.

Fig. 14.1
14.2) or front coarse feed (Fig. 14.3).

The model with side coarse feed can be delivered with

the coarse advance movement of the specimen to-
wards the knife being effected either by clockwise
(CW) or by counterclockwise (CCW) rotation.

The function is the same on both versions. The coarse

feed operates the rapid horizontal specimen movement
forward - towards the knife - and backward - away
from the knife by turning the coarse feed wheel in the
corresponding direction.

Once it becomes hard to turn the coarse feed wheel,

the front or rear limit is reached. When the front limit is
Fig. 14.2
reached, no further advance will take place.

The coarse feed wheel rotates while section-

ing by turning the handwheel!
Therefore, make sure that the coarse feed
wheel can rotate freely!
If obstructed, no section thickness feed takes
place and thus no section will be obtained.

Fig. 14.3

14 Instruction manual V 1.1 – 02/2001

8. Operation

8.3 Specimen retraction

The specimen retraction protects the knife and speci-
men from damage.

When using the specimen retraction feature, the

sample is retracted by 200 µm after the sectioning
stroke while travelling upward and returning to the
starting position. Before the sample advances by the
selected section thickness, a feed by the retraction
value takes place.

The specimen retraction feature can be deactivated

when required via the screw at the rear of the instru-
ment by using the screw driver provided.

Prior to activating or deactivating the specimen retrac-

tion, move the specimen head in the top position of the
stroke by turning the handwheel.

• To deactivate, position the slot of the screw hori-

zontal.
= Retraction ON
• To activate, position the slot of the screw vertical.
= Retraction OFF

Fig. 15.1 8.4 Mechanical trimming function (optional)

The RM 2135 is available with and without mechanical
trimming function. The trimming function is activated
by means of the trimming lever.

The trimming lever has three locking positions (off, 10

µm, 30 µm). The trimming increments are marked by
dots:

l = 10 µm
l l= 30 µm

• To activate the trimming function, push the lever

and hold it down in one of the locking positions.

Each rotation of the handwheel will then cause a speci-

men advance of 10 µm or 30 µm.

Once released, the lever automatically flips back into

Fig. 15.2
its off-position. The trimming function will then be de-
activated.

The selected section thickness will not be

added to the trimming value.
If the section thickness value is greater than
the trimming value, the sample will advance
by the section thickness.

Leica RM 2135 – Rotary Microtome 15

9. Specimen clamping system

The handling of the specimen clamps and

The specimen clamping system accommodates all
specimen holders is described in chapter 15 specimen clamps which are available as accessories.
Accessories. The specimen clamping system is available in a non-
orienting and an orienting version. Both systems are
exchangeable.

The specimen orientation feature allows for an easy

correction of the position of the specimen surface
when the specimen is fixed in the clamp.

2
1

9.1 Mounting the specimen clamp

• Rotate the handwheel to position the specimen
clamping system at the top of the stroke and acti-
vate the handwheel brake.

• To release, turn the clamping lever (1) counterclock-

wise.

• Insert the dovetail guide (2) of the specimen clamp

into the dovetail adapter (3) from the left.

• To clamp, turn the clamping lever (1) clockwise to

the limit stop.
3
Fig. 16.1

9.2 Orienting the specimen

• Rotate the handwheel to position the specimen
clamping system at the top of the stroke and acti-
vate the handwheel brake.

• To release, turn the eccentric bolt (4) counterclock-

wise.

• To orient the sample in north/south direction, turn

the adjusting screw (5) . To orient it in east/west
direction use the adjusting screw (6) .

• To fix the orientation, turn the eccentric bolt (4)

6 clockwise.
Fig. 16.2

16 Instruction manual V 1.1 – 02/2001

10. Universal knife holder base

10.1 Knife holder base with lateral

displacement
The knife holder base with lateral displacement func-
tion consists of two parts and therefore allows for
both north/south and east/west displacement.

Fig. 17.1

10.2 Knife holder base without lateral

displacement
The knife holder base without lateral displacement
can only be repositioned forth and back on the base
plate of the microtome.
Fig. 17.2

10.3 Repositioning the knife holder base

The plastic grips of all clamping levers can North/south
be repositioned as preferred by the individual
user. North/south displacement enables the approach of
Pull the grip out of the lever, hold it in this po- the knife holder to the sample as close as possible.
sition, and turn it to the desired position. It will
then lock automatically when released. • To release, turn the clamping lever (1) on the right
side of the microtome base plate counterclock-
wise.
• Reposition the knife holder base together with the
knife holder forward or backward as appropriate.
• To clamp, turn the clamping lever (1) clockwise.

East/west (applies only to 10.1)

The lateral displacement feature of the knife holder
base enables the use of the entire length of the blade
or knife edge eliminating the need for readjusting the
2
knife holder.

• To release, turn the clamping lever (2 not visible

in Fig. 17.3) on the left of the knife holder clock-
wise.
• Reposition the knife holder base together with the
1 knife holder sideways as required.
• To secure, turn the clamping lever (2) counter-
clockwise.

Fig. 17.3

Leica RM 2135 – Rotary Microtome 17

10. Universal knife holder base

The handling of the various knife holders is 10.4 Mounting the knife holder
described in chapter 15 Accessories.
• To release, turn the clamping lever (1) counterclock-
wise.

• Slide the guide of the knife holder (2) onto the T

piece (3) of the knife holder base.
2 3
• To lock, turn the clamping lever (1) clockwise.

1
Fig. 18.1

10.5 Adjusting the clearance angle

The scale for the clearance angle adjustment (0°, 5°
and 10°) is located on the right side of the knife holder.
Moreover, a reference line is provided on the right side
of the knife holder base to adjust the angle.

• To release, turn the clamping lever (1) counterclock-

wise.
1

• To adjust the clearance angle, slide the knife holder

on the base until the reference line on the knife
holder base is located below the scale line for the
Fig. 18.2
required adjustment.

• Hold the knife holder in this position and fix the ad-
justment by turning the lever (1) clockwise.

Fig. 18.3

18 Instruction manual V 1.1 – 02/2001

11. Daily operation

Description of the individual steps using the • Start trimming by turning the handwheel.
paraffin sectioning technique • Stop trimming upon raching the desired sectioning
plane.
• Release the trimming lever.
Always lock the handwheel prior to manipu-
• Select the appropriate section thickness or verify
lating the knife and specimen or before chang-
ing the specimen or knife!
the value previouslyselected.

• Lock the handwheel. Make sure that the coarse feed wheel can
rotate freely!
Always clamp the specimen before the knife! If obstructed, no section thickness feed takes
place and thus no section will be obtained.
• Mount the precooled paraffin block in the univer-
• Rotate the handwheel clockwise at a uniform
sal cassette clamp.
speed.
Take care when handling microtome knives • Prepare the sections.
and disposable blades. The cutting edge is
extremely sharp and can cause severe injury!

• Push the knife guard to the middle of the knife Changing the specimen or interrupting
holder. sectioning
• Insert the blade into the knife holder and clamp.
• Adjust the appropriate clearance angle (try an Prior to manipulating the knife and specimen,
angla between 0 and 3°). or changing the specimen or knife, and dur-
• Move the specimen to the rear limit by turning the ing breaks, always lock the handwheel and
coarse feed in the appropriate direction. cover the cutting edge with the knife guard!

Do not attempt to clamp, approach or orient • Lock the handwheel.

the specimen in the retraction phase! • Position the knife guard over the cutting edge.
Prior to the subsequent section a specimen • Remove the specimen from the specimen clamp and
advance will be effected by the retracted mount a new specimen to continue.
value plus the selected thickness.
This involves the risk that the specimen col-
lides with the knife!
Terminating sectioning
• Approach the knife holder on the knife holder base • Lock the handwheel.
to the spacimen as close as possible. • Remove the blade from the knife holder and put it in
• Orient the specimen surface towards the knife. the receptacle at the bottom of the dispenser.
• Remove the specimen from the specimen clamp.
• Pull the section waste tray (if available) out. • Transfer all section waste to the section waste tray
• Remove the knife guard from the blade. if available.
• Unlock the handwheel. • Empty the section waste tray.
• Hold the trimming lever down to select the appro- • Clean the instrument (see chapter 13 - 'Cleaning').
priate trimming value.

Rotate the handwheel at a speed that suits the

hardness of the specimen!
Always rotate at a slow speed when section-
ing hard samples.

Leica RM 2135 – Rotary Microtome 19

12. Trouble shooting

12.1 Problems, possible causes and corrective action

Problem Cause Corrective action

Thick/thin sections - Insufficient knife/blade inclina- - Systematically try several

The section thickness varies from tion; consequently the clear- clearance angle adjustments,
one section to another. In extreme ance angle is too small. until the optimum angle is
cases, sections are skipped found.
meaning that a section is not
obtained. - Insufficient clamping of speci- - Check if all levers are locked
men and/or knife. and screws are tightened on
the specimen and knife holder
systems. Retighten the levers
and screws if necessary.

- Blunt knife/blade. - Use a different part of the

cutting edge or use a new knife/
blade.

Compressed sections - Blunt knife/blade. - Use a different part of the

The sections are extremely cutting edge or use a new
compressed, wrinkled or jammed knife/blade.
together.
- Specimen too warm. - Precool the specimen on a cold
plate.
- Cool the specimen in iced water
immediately before sectioning.

- Clearance angle too big. - Clearance angle adjustment;

systematically decrease the
clearance angle until the
optimum adjustment is ob-
tained.

- Sectioning speed too high. - Rotate the handwheel at a

lower speed.

The knife "rings" on the cutting - Sectioning speed too high. - Rotate the handwheel at a
stroke when sectioning hard lower speed.
specimens. Sections exhibit
scratches and chatter marks. - Clearance angle too big. - Clearance angle adjustment;
systematically decrease the
clearance angle until the
optimum adjustment is ob-
tained.

- Insufficient clamping of speci- - Check if all levers are locked

men and/or knife. and screws are tightened on
the specimen and knife holder
systems. Retighten the levers
and screws if necessary.

20 Instruction manual V 1.1 – 02/2001

12. Trouble shooting

12.2 Instrument errors, possible causes and corrective action

Problem Possible Cause Corrective action

No more specimen advance 1. Specimen reached the front 1. Turn the coarse feed wheel in
and consequently no section feed limit. the appropriate direction to
produced. move the specimen towards the
rear limit.

2. The coarse feed wheel cannot 2. Remove any obstruction.

rotate freely.

Leica RM 2135 – Rotary Microtome 21

13. Cleaning

Lock the handwheel before cleaning the in-

strument!

• Remove all debris with a dry brush.

• Remove the section waste tray (if available) for
emptying.
• Remove the arm rests for cleaning.
• Remove the knife holder and knife holder base for
cleaning.

Only use mild commercial detergents or soap

solution for cleaning!
Do not use solvents that contain acetone and
xylene!

Ensure that no liquids enter the interior of the

instrument when cleaning!

• Clean the instrument and accessory components

with a damp not a wet cloth.

22 Instruction manual V 1.1 – 02/2001

14. Maintenance

14.1 General maintenance

The microtome is virtually maintenance-free. To en-
sure a smooth operation of the instrument over sev-
1 eral years we recommend the following:

• Have the instrument inspected once a year by a

qualified service engineer authorized by Leica.

• Enter into a service contract at the end of the war-

ranty period.
For further information, please contact your local
Leica service center.

• Clean the instrument every day.

2 • Lubricate the following parts with the oil No. 405

Fig. 23.1 once a month (1- 2 drops will be sufficient):

- T piece (1) on the microtome base plate.

- Guides (2) of the knife holder base on the micro-
3
tome base plate.
5 - Clamping levers (3) and (4) on the right and left
of the knife holder base.
- T piece (5) on the knife holder base.
4
• Do not carry out any repairs on your own as this
will invalidate the warranty.
Repairs may only be carried out by qualified ser-
vice engineers authorized by Leica.
Fig. 23.2

Leica RM 2135 – Rotary Microtome 23

14. Maintenance

14.2 Adjusting the spring lever system

When using different specimen clamps and samples
of various sizes and weights, it may be necessary to
readjust the counterbalance weight.

How to check correct adjustment

• Fix the specimen clamp with the sample.

• Turn the handwheel to position the sample at half

the height of the vertical stroke.

If the specimen head remains in this position without

travelling upward or downward, the adjustment is cor-
rect.

Readjustment will be required if the specimen head

slightly rises or drops.

How to readjust
The adjustment is made with the screw that is acces-
sible through the hole on the underside of the micro-
tome base plate, after having removed the section
waste tray if used, by means of the screw driver pro-
vided.

• If the specimen head slightly rises, turn the screw

counterclockwise completing two or three full ro-
tations.

• If the specimen head slightly drops, turn the screw

clockwise completing two or three full rotations.

Fig. 24 Check for correct adjustment after each adjusting step.

Repeat the adjustment several times if required.

24 Instruction manual V 1.1 – 02/2001

15. Accessories

15.1 Specimen clamps

All specimen clamps can be used in combi-

nation with both the orienting and non-orient-
ing specimen clamping system.

15.1.1 Standard specimen clamp

1 The standard specimen clamp is available in two sizes:
40 x 40 mm and 50 x 55 mm.
It is designed for direct clamping of rectangular blocks.
In addition, it accommodates the foil clamps.

2 • Turn the knurled knob (1) counterclockwise to move

the movable jaw (2) downward.

• Mount the sample as required.

• Turn the knurled knob (1) clockwise to move the

movable jaw (2) upward against the fixed jaw to se-
curely clamp the sample.
Fig. 25.1

15.1.2 Universal cassette clamp

3
The universal cassette clamp (UCC) is designed to for
horizontal or vertical clamping of all kinds of commer-
cial cassettes.

• Pull the lever (3) to the front.

• Mount the cassette horizontally or vertically as re-

quired.

• Release the lever (3) to secure the cassette in po-

sition.

Fig. 25.2

Leica RM 2135 – Rotary Microtome 25

15. Accessories

15.1.3 Holder for round specimens

4 9
The holder for round specimens is designed to accom-
modate cylindrical samples. Inserts for specimens of
6, 15 and 25 mm diameter are available.
3
• To mount the required insert (2, 3, 4) turn the clamp-
ing ring (1) counterclockwise and remove.

• Place the insert (2), (3) or (4) in the clamping ring (1)
and fix the clamping ring (1) by turning it clockwise.

2 • To mount the specimen, turn the clamping ring (1)

1
counterclockwise, mount the sample (5) and fix by
turning the clamping ring clockwise.

• To orient the sample, introduce the pin (9) in one of

5 the holes (10) and turn counterclockwise.

10 • To fix the orientation, introduce the pin (9) again in

one of the holes (10) and turn clockwise.

Fig. 26.1
15.1.4 Vee insert
1
The vee insert is mounted in the hole provided in the
lower movable jaw of the standard specimen clamp.
This enables to clamp round specimens in the stan-
dard specimen clamp.

• Turn the knurled knob (1) counterclockwise to move

5
2
the movable jaw (2) downward.

• Insert the pin (3) of the vee insert (4) in the hole (5)
4 of the lower jaw (2).

• Mount the sample as required.

• Turn the knurled knob (1) clockwise to move the

movable jaw (2) with the vee insert (4) upward
against the fixed jaw to securely clamp the sample.
3
Fig. 26.2

26 Instruction manual V 1.1 – 02/2001

15. Accessories

15.1.5 Foil clamp - Type 1

This foil clamp is appropriate both for clamping very
small foil pieces and flat, angular samples. It is mounted
in the standard specimen clamp..

1
Clamping of foil pieces
• Move the movable jaw (3) to the right or left as re-
quired by turning the set screw with an Allen key
size 4 (6).
4
3 • Place the foil (2) between the movable jaw (3) and
the fixed jaw (4).

• To clamp the foil, screw the movable jaw (3) against

the fixed jaw (4) by using the Allen key (6).

• Insert the foil clamp in the standard specimen clamp

as shown.

• Turn the knurled knob (1) clockwise to securely

clamp the foil clamp in the standard specimen
clamp.
2
6
Fig. 27.1 Clamping of flat samples
1 To clamp flat samples, replace the long set screw (5)
with the short set screw (7) provided with the foil clamp.

• Unscrew the long set screw (5) on the left with an

Allen key size 4.

• Screw the short set screw (7) in the hole on the right.
4
5 3
• Place the sample (2) between the movable jaw (3)
and the fixed jaw (4).

• To clamp the sample, screw the movable jaw (3)

against the fixed jaw (4).

• Insert the foil clamp in the standard specimen clamp

as shown.

• Turn the knurled knob (1) clockwise to securely

7 clamp the foil clamp in the standard specimen
2 clamp.
Fig. 27.2

Leica RM 2135 – Rotary Microtome 27

15. Accessories

15.1.6 Foil clamp - Type 2

This foil clamp is appropriate for large foil ribbons and
is mounted in the standard specimen clamp.
1
• To open the jaws (3) and (4) lightly loosen the 3
screws (5) with an Allen key size 4 (6).
2
7
• Insert the foil (2) from behind to position it between
the movable jaw (3) and the fixed jaw (4).
4 3
• To clamp the foil, first tighten the screw (5) in the
middle and then the other two screws with the Allen
key.

• Place the foil clamp in the standard specimen clamp

so that the bevelled surface (7) on the back of the
foil clamp points to the right or left.

• Turn the knurled knob (1) clockwise to securely

clamp the foil clamp in the standard specimen
5 clamp.

Fig. 28

28 Instruction manual V 1.1 – 02/2001

15. Accessories

15.2 Knife holders

Knife guard

The safety devices on the instrument and ac-

2 cessory equipment must not be removed or
1 modified!
2
Every knife holder has a permanent, movable knife
guard (1) that allows to cover the cutting edge com-
pletely in any position of the knife.
Grips (2) are provided to conveniently move the knife
guard. Push both parts of the knife guard to the middle
to cover the knife edge.

Prior to manipulating the knife and specimen,

or changing the specimen or knife, and dur-
Fig. 29.1
ing breaks, always lock the handwheel and
cover the cutting edge with the knife guard!
2
1

Microtome knives and disposable blades

Take care when handling microtome knives

and disposable blades. The cutting edge is
extremely sharp and can cause severe injury!

Never leave knives and knife holders with a

mounted knife or blade lying around!
Fig. 29.2
Always put the knives back into the knife case
when not in use!

Do not place a knife on a table with the cut-

ting edge facing upward!

Never try to catch a falling knife!

Cleaning

Only use mild commercial detergents or soap

solution for cleaning!
Do not use solvents that contain acetone and
xylene!

• Clean the knife holders with a damp not a wet cloth.

Leica RM 2135 – Rotary Microtome 29

15. Accessories

15.2.1 Knife holder N

This knife holder is designed for standard steel and
tungsten carbide knives, profile c and d, up to 16 cm
long. In addition, it is appropriate for various blade rails
for disposable blades, which are inserted in the holder
like a knife.
The knife can be repositioned in height. This enables
the use even of frequently resharpened knives.

Mounting the knife support bar

• Push the knife guard (1) to the middle.
• Place the knife support bar (3) as shown on the
1 height adjustment screws (not visible), ensuring that
1
the flat top ends of the height adjustment screws
are seated in the slots at both ends of the knife sup-
4
port bar.

Inserting the knife

• Turn the knurled wheels (6) on the right and left of
the knife holder in opposite directions to the front
to move the knife support bar via the height adjust-
ment screws to the lowest position, to prevent dam-
age to the cutting edge while inserting the knife.
2
• Unscrew the knife clamping screws (2) counter-
6 3
clockwise as far out as possible.
• Hold the knife (4) at the knife back and carefully in-
Fig. 30.1 sert it in the holder from the side as shown with the
cutting edge facing upward.

Adjusting the knife height

When adjusting the clearance angle, the cutting edge
of the knife should be positioned in the center of rota-
tion of the knife holder. The knife is correctly positioned
in height when the cutting edge is at the level of the
locating edge of the rear clamping jaws. The knife edge
should be parallel with the locating edges.

• Turn the knurled wheels (6) in opposite directions

to the rear to position the knife edge parallel and at
the level der of the locating edge (Fig. 30.2) of the
rear clamping jaws.
• To clamp the knife (4), tighten the two clamping
screws (2) uniformly by turning them clockwise.

Inserting the blade rails

Fig. 30.2 The blade rails are inserted in the knife holder and
clamped like a knife.

30 Instruction manual V 1.1 – 02/2001

15. Accessories

15.2.2 Knife holder NZ

The knife holder NZ is appropriate for standard steel
and tungsten carbide knives, profile c and d, up to 16
cm long. With its central clamping feature, the knife
can be laterally repositioned and enables the use of
the full length of the cutting edge. The knife can be
repositioned in height. This enables the use even of
frequently resharpened knives.

Mounting the knife support bar

• Push the knife guard (1) to the middle.
1 • Place the knife support bar (3) as shown on the
1
height adjustment screws (not visible), ensuring that
the flat top ends of the height adjustment screws
4
are seated in the slots at both ends of the knife sup-
port bar.

Inserting the knife

• Turn the knurled wheels (6) on the right and left of
the knife holder in opposite directions to the front
to move the knife support bar via the height adjust-
ment screws to the lowest position, to prevent dam-
2
age to the cutting edge while inserting the knife.
6 3
• Unscrew the knife clamping screws (2) counter-
clockwise as far out as possible.
Fig. 31 • Hold the knife (4) at the knife back and carefully in-
sert it in the holder from the side as shown with the
cutting edge facing upward.

Adjusting the knife height

Please refer to 15.5.1 Knife holder N (Fig. 30.2).

Lateral repositioning of the knife

• Push the knife guard (1) to the middle.
• Loosen the clamping screws (2) by turning them
counterclockwise.
• Push the knife (4) to the left or right as required.
• To clamp the knife (4), always tighten the clamping
screw (2) first which is located on the side to which
the knife has been repositioned by turning it clock-
wise.

Leica RM 2135 – Rotary Microtome 31

15. Accessories

15.2.3 Knife holder E

The knife holder E is designed for conventional dispos-
able blades from different manufacturers.
It is available in two versions:
1 5
With pressure plate for low profile blades and with
pressure plate for high profile blades.
Both pressure plates also are available as accesso-
ries. If you would like to change from one blade type to
another, just order the required type of pressure plate.
The pressure plate can be exchanged easily.
4
3
Inserting the blade
• Push the knife guard (1) to the middle.
• To insert the blade, relocate the clamping lever (2)
down.
• Carefully insert the blade (3) from the side.
• To clamp the blade, relocate the clamping lever (2)
up.
2
Fig. 32.1 Changing the pressure plate
• Loosen the 4 screws (4) on the back of the knife
holder by using an Allen key size 4.
• Remove the pressure plate (5).
• Attach the new pressure plate by tighten the 4
screws (4).
7
Cleaning
5
The clamping plate (7) can be removed for cleaning.

2 • To remove the blade, relocate the clamping lever

(2) down.
• Carefully remove the blade.
• Pull out the clamping lever (2).
• Remove the clamping plate (7).

Only use mild commercial detergents or soap

Fig. 32.2 solution for cleaning!
Do not use solvents that contain acetone and
xylene!

• Clean the knife holder with a damp not a wet cloth.

• Place the clamping plate (7) on the holder ensuring
that the upper edge of the clamping plate and of
Convenient for left-handers:
the pressure plate (5) are parallel and at the same
The clamping lever (2) can also be inserted in
the hole on the left side of the knife holder. height.
This will however change the locking posi- • Insert the clamping lever (2) in the hole and relo-
tion of the lever! cate it up.
To unlock, relocate the lever upward; to
clamp, relocate the lever downwards.

32 Instruction manual V 1.1 – 02/2001

15. Accessories

15.2.4 Knife holder E-TC

The knife holder E-TC is designed for the Leica TC-65
tungsten carbide blades.

1
Inserting the blade
1 • Push the knife guard (1) to the middle.
• To insert the blade, relocate the clamping lever (2)
down.
3 • Carefully insert the tungsten carbide blade (3) from
the side with the shining facet to the front.
• To clamp the blade, relocate the clamping lever (2)
up.

Cleaning
The clamping plate (7) can be removed for cleaning.
Fig. 33.1 2
• To remove the blade, relocate the clamping lever
(2) down.
• Carefully remove the blade.
7
• Pull out the clamping lever (2).
5 • Remove the clamping plate (7).

Only use mild commercial detergents or soap

2
solution for cleaning!
Do not use solvents that contain acetone and
xylene!

• Clean all components with a damp not a wet cloth.

• Place the clamping plate (7) on the holder ensuring
that the upper edge of the clamping plate and of
the pressure plate (5) are parallel and at the same
Fig. 33.2
height.
• Insert the clamping lever (2) in the hole and relo-
cate it up.

Leica RM 2135 – Rotary Microtome 33

15. Accessories

15.3 Section waste tray

The section waste tray is a handy accessory

for instruments with lateral coarse feed
wheel.
When using it with the front coarse feed
model, the waste tray will have to be pulled
out of the base plate very far in order to en-
able convenient turning of the coarse feed
wheel.

• Insert the section waste tray under the microtome

base plate at the front.

Fig. 34

34 Instruction manual V 1.1 – 02/2001

15. Accessories

15.4 Hand rest

The hand rest is accompanied by two long

clamping levers for the knife holder base and
a long trimming lever for instruments with
mechanical trimming function (option).

Exchanging the trimming lever

(on instruments with mechanical trimming function)
• Pull the short trimming lever out of the holding de-
vice.

• Insert the long trimming lever into the slot and push
it until it engages in the holding device.

Mounting the hand rest

Prior to mounting the hand rest, the short clamping
lever(s) - depending on the knife holder base type you
are using - has/have to be removed from the knife
holder base
1
• To release, turn the lever (1) counterclockwise. To
release turn the lever (2) (on knife holder base with
2 lateral displacement only) clockwise.
5
Pull both levers out of the knife holder base.

The knife holder base without lateral dis-

placement has hole on the left side which is
covered with a plastic cap. The cap can be
removed with a screw driver. The hole will
4 accommodate a second clamping lever to
stabilise the hand rest.

• Place the hand rest (3) on the microtome base plate.

• Insert the longer lever (4) and the shorter lever (5)
3 through the holes on the right and left of the hand
Fig. 35 rest in the holes of the knife holder base and lock
both levers.

Leica RM 2135 – Rotary Microtome 35

15. Accessories

Repositioning the knife holder base

The hand rest and knife holder do not need to

be removed as they are repositioned in both
directions together with the knife holder base.

North/South
• To release, turn the clamping lever (1) on the left
side of the microtome base plate counterclockwise.
• Reposition the knife holder together with the knife
holder base and hand rest forward or backward as
2 appropriate.
• To clamp, turn the clamping lever (1) clockwise.

East/West (applies only to knife holder base with

lateral displacement)
• To release, turn the clamping lever (2, not visible
1 here) on the left of the hand rest clockwise.
3 • Reposition the knife holder together with the hand
rest and knife holder base sideways as required.
• To secure, turn the clamping lever (2) counterclock-
Fig. 36.1 wise.

Adjusting the clearance angle

The scale for the clearance angle adjustment (0°, 5°
and 10°) is located on the right side of the knife holder.
A reference point is provided on the hand rest.

• To release, turn the clamping lever (3) counterclock-

wise.
• To adjust the clearance angle, slide the knife holder
on the base until the reference point on the hand
rest is located next to the scale line for the required
adjustment.
• Hold the knife holder in this position and fix the ad-
justment by turning the lever (3) clockwise.

3
Fig. 36.2

36 Instruction manual V 1.1 – 02/2001

16. Ordering information

RM 2135 Rotary Microtome - Basic instrument

with mechanical trimming device, side coarse feed, clockwise ...................................................................... 0500 29802
without mechanical trimming device, side coarse feed, clockwise ................................................................ 0500 29134
with mechanical trimming device, side coarse feed, counterclockwise ........................................................ 0500 29135
without mechanical trimming device, side coarse feed, counterclockwise .................................................. 0500 29136
with mechanical trimming device, front coarse feed, clockwise ..................................................................... 0500 29137
without mechanical trimming device, front coarse feed, clockwise ............................................................... 0500 29138

Knife holder system

Universal knife holder base with lateral displacement ...................................................................................... 0500 29703
Universal knife holder base without lateral displacement ................................................................................. 0500 30147
Knife holder N ............................................................................................................................................................ 0502 29955
Knife holder NZ .......................................................................................................................................................... 0502 29956
Knife holder E for low profile blades ...................................................................................................................... 0502 29957
Pressure plate for high profile blades .................................................................................................................... 0502 29553
Knife holder E for high profile blades ..................................................................................................................... 0502 29982
Pressure plate for low profile blades ..................................................................................................................... 0502 29551
Knife holder E-TC ....................................................................................................................................................... 0502 29958
Knife holder GD for glass and diamond knives ..................................................................................................... 0502 29959

Specimen holder system

Specimen clamping system, non-orienting ........................................................................................................... 0502 29963
Specimen clamping system, orienting ................................................................................................................... 0502 29962
Standard specimen clamp with adapter (50 x 55 mm) ......................................................................................... 0502 29964
Standard specimen clamp with adapter (40 x 40 mm) ......................................................................................... 0502 29980
Universal cassette clamp with adapter ................................................................................................................. 0502 29965
Vee insert for round specimens .............................................................................................................................. 0402 09299
Foil clamp, Type I ....................................................................................................................................................... 0402 09307
Foil clamp, Type II ...................................................................................................................................................... 0402 26922
Holder for round specimens with adapter with 3 inserts .................................................................................... 0502 29979
Holder for round specimens with adapter without inserts ................................................................................. 0502 29522
Insert for holder for round specimens, ø 6 mm ..................................................................................................... 0356 08322
Insert for holder for round specimens, ø 15 mm ................................................................................................... 0356 09200
Insert for holder for round specimens, ø 25 mm ................................................................................................... 0356 08320
Holder for EM specimen holder .............................................................................................................................. 0502 29968
Segment arc with adapter for EM specimen holder ............................................................................................ 0502 29969

Accessories
Section waste tray .................................................................................................................................................... 0500 29807
Hand rest including long clamping levers and long lever for the trimming device ........................................ 0500 30143

Accessories included in standard delivery for reordering

Arm rest, right ............................................................................................................................................................ 0500 29608
Arm rest, left ............................................................................................................................................................... 0500 29609
Maintenance kit ......................................................................................................................................................... 0500 30144
Cover cap .................................................................................................................................................................... 0430 18995
Dust cover ................................................................................................................................................................... 0212 04091
Instruction manual V 1.1 – 02/2001 (German, English, French, Spanish) .......................................................... 0700 37103

Leica RM 2135 – Rotary Microtome 37

17. Appendix

Product changes resentative or Dealer, or Leica Microsystems Nussloch

GmbH in Germany.
Due to a policy of continuous improvement of our prod- We will be glad to provide you with details on the re-
ucts, Leica Microsystems Nussloch GmbH reserves the cycling concept for our microtomes meeting today's
right to change specifications without notice. environmental requirements.

Warranty Technical service information

Leica Microsystems Nussloch GmbH guarantees that If you require technical service or replacement parts
the delivered product has been subjected to a com- under warranty, please contact your Leica Sales Rep-
prehensive quality control procedure based on our resentative or Dealer from whom the instrument was
strict in-house testing standards in order to ensure that purchased.
the product complies with its technical specification. Be sure to state the model type, serial number and date
The warranty conditions depend on the contents of the of delivery. Leica Microsystems Nussloch GmbH (Ger-
individual contract concluded, supplemented by the many) cannot accept goods returned without official
warranty conditions of your local Leica sales agency. authorization.
Any repairs and/or exchange of parts of the product If an instrument or any part of it is to be returned to
must be carried out by authorized Leica technical ser- Leica, please note the following:
vice engineers. Otherwise, any warranty becomes in-
valid and warranty claims can no longer be made. a. If the instrument or any part of it has been exposed
The local Leica representative or the manufacturer in to or been in contact with potentially pathogenic or
Nussloch must be consulted prior to any handling of radioactive materials, it is essential to decontami-
or changes to the instrument beyond the scope of this nate the instrument or part.
instruction manual as well as prior to any modifica- Decontamination must explicitly be confirmed by
tions or any use of the instrument in combination with the customer. Our service engineers have to en-
non-Leica components not expressly authorized by quire about this.
Leica.
Spare parts and accessories not supplied by Leica can b. Ensure that there is no radioactivity or hazardous
under no circumstances be considered as inspected bacteria present and advise Leica of any decon-
and/or approved by Leica. tamination procedure that may have been carried
Therefore, installation or use of any such parts may out.
impair the technical design features and thus proper-
ties of the instrument. Should the instrument or any part of it be received in
a condition that Leica considers to be a potential bio-
Leica assumes no liability whatsoever for any logical hazard, the instrument or part will be returned
damage caused by the use of non-original unrepaired at the expense of the customer.
spare parts or non-original accessories.
When requesting a service call, please provide the fol-
The warranty is only valid and warranty claims can only lowing information:
be made as long as the instrument has been operated
according to its designated use and according to the a. Model type and serial number of the instrument;
instructions given in this manual.
Improper use of the product and/or faulty operation b. Location of the instrument and the person to con-
invalidate the warranty and any claims based thereon, tact;
and likewise Leica will not assume liability for any con-
sequential damage. c. The reason for the service call.

Disposal
The instrument or parts of the instrument must be dis-
posed of in compliance with the local laws.
The RM 2135 contains a great number of recyclable
components. For more information about our recycling
program, please contact your local Leica Sales Rep.

38 Instruction manual V 1.1 – 02/2001

Notes

Leica RM 2135 – Rotary Microtome 39

Notes

40 Instruction manual V 1.1 – 02/2001