To advance the utilization of microbial cell factories in gas fermentation processes, their physiological and biotechnological characteristics must be understood. Here, the authors report on the construction and operation of a novel device, the Gas and Pressure Controller (GPC), which is specifically designed for the automated control of the headspace gas pressure of closed cultivation bottles and facilitates automated gassing, sparging, monitoring and regulation of the headspace volume operated in closed batch cultivation mode in real time.
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